Quantitative and qualitative preparations of bacterial outer membrane vesicles
Publication
, Journal Article
Chutkan, H; MacDonald, IA; Manning, AJ; Kuehn, MJ
Published in: Methods in Molecular Biology
December 1, 2013
Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains. © Springer Science+Business Media New York 2013.
Duke Scholars
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
December 1, 2013
Volume
966
Start / End Page
259 / 272
Related Subject Headings
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Chutkan, H., MacDonald, I. A., Manning, A. J., & Kuehn, M. J. (2013). Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology, 966, 259–272. https://doi.org/10.1007/978-1-62703-245-2-16
Chutkan, H., I. A. MacDonald, A. J. Manning, and M. J. Kuehn. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods in Molecular Biology 966 (December 1, 2013): 259–72. https://doi.org/10.1007/978-1-62703-245-2-16.
Chutkan H, MacDonald IA, Manning AJ, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology. 2013 Dec 1;966:259–72.
Chutkan, H., et al. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods in Molecular Biology, vol. 966, Dec. 2013, pp. 259–72. Scopus, doi:10.1007/978-1-62703-245-2-16.
Chutkan H, MacDonald IA, Manning AJ, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology. 2013 Dec 1;966:259–272.
Published In
Methods in Molecular Biology
DOI
ISSN
1064-3745
Publication Date
December 1, 2013
Volume
966
Start / End Page
259 / 272
Related Subject Headings
- Developmental Biology
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences