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Quantitative and qualitative preparations of bacterial outer membrane vesicles

Publication ,  Journal Article
Chutkan, H; MacDonald, IA; Manning, AJ; Kuehn, MJ
Published in: Methods in Molecular Biology
December 1, 2013

Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains. © Springer Science+Business Media New York 2013.

Duke Scholars

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

December 1, 2013

Volume

966

Start / End Page

259 / 272

Related Subject Headings

  • Developmental Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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Chutkan, H., MacDonald, I. A., Manning, A. J., & Kuehn, M. J. (2013). Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology, 966, 259–272. https://doi.org/10.1007/978-1-62703-245-2-16
Chutkan, H., I. A. MacDonald, A. J. Manning, and M. J. Kuehn. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods in Molecular Biology 966 (December 1, 2013): 259–72. https://doi.org/10.1007/978-1-62703-245-2-16.
Chutkan H, MacDonald IA, Manning AJ, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology. 2013 Dec 1;966:259–72.
Chutkan, H., et al. “Quantitative and qualitative preparations of bacterial outer membrane vesicles.” Methods in Molecular Biology, vol. 966, Dec. 2013, pp. 259–72. Scopus, doi:10.1007/978-1-62703-245-2-16.
Chutkan H, MacDonald IA, Manning AJ, Kuehn MJ. Quantitative and qualitative preparations of bacterial outer membrane vesicles. Methods in Molecular Biology. 2013 Dec 1;966:259–272.

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

December 1, 2013

Volume

966

Start / End Page

259 / 272

Related Subject Headings

  • Developmental Biology
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences