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Detection of autofluorescent Mycobacterium chelonae in living zebrafish.

Publication ,  Journal Article
Whipps, CM; Moss, LG; Sisk, DM; Murray, KN; Tobin, DM; Moss, JB
Published in: Zebrafish
February 2014

Mycobacterium chelonae is widespread in aquatic environments and can cause mycobacteriosis with low virulence in zebrafish. The risk of infection in zebrafish is exacerbated in closed-recirculating aquatic systems where rapidly growing mycobacteria can live on biofilms, as well as in zebrafish tissues. We have discovered a method of identifying and visualizing M. chelonae infections in living zebrafish using endogenous autofluorescence. Infected larvae are easily identified and can be excluded from experimental results. Because infection may reduce fertility in zebrafish, the visualization of active infection in contaminated eggs of transparent casper females simplifies screening. Transparent fish are also particularly useful as sentinels that can be examined periodically for the presence of autofluorescence, which can then be tested directly for M. chelonae.

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Published In

Zebrafish

DOI

EISSN

1557-8542

Publication Date

February 2014

Volume

11

Issue

1

Start / End Page

76 / 82

Location

United States

Related Subject Headings

  • Zebrafish
  • Optical Imaging
  • Mycobacterium chelonae
  • Mycobacterium Infections, Nontuberculous
  • Larva
  • Fluorescence
  • Fish Diseases
  • Female
  • Developmental Biology
  • Animals
 

Citation

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Whipps, C. M., Moss, L. G., Sisk, D. M., Murray, K. N., Tobin, D. M., & Moss, J. B. (2014). Detection of autofluorescent Mycobacterium chelonae in living zebrafish. Zebrafish, 11(1), 76–82. https://doi.org/10.1089/zeb.2012.0863
Whipps, Christopher M., Larry G. Moss, Dana M. Sisk, Katrina N. Murray, David M. Tobin, and Jennifer B. Moss. “Detection of autofluorescent Mycobacterium chelonae in living zebrafish.Zebrafish 11, no. 1 (February 2014): 76–82. https://doi.org/10.1089/zeb.2012.0863.
Whipps CM, Moss LG, Sisk DM, Murray KN, Tobin DM, Moss JB. Detection of autofluorescent Mycobacterium chelonae in living zebrafish. Zebrafish. 2014 Feb;11(1):76–82.
Whipps, Christopher M., et al. “Detection of autofluorescent Mycobacterium chelonae in living zebrafish.Zebrafish, vol. 11, no. 1, Feb. 2014, pp. 76–82. Pubmed, doi:10.1089/zeb.2012.0863.
Whipps CM, Moss LG, Sisk DM, Murray KN, Tobin DM, Moss JB. Detection of autofluorescent Mycobacterium chelonae in living zebrafish. Zebrafish. 2014 Feb;11(1):76–82.
Journal cover image

Published In

Zebrafish

DOI

EISSN

1557-8542

Publication Date

February 2014

Volume

11

Issue

1

Start / End Page

76 / 82

Location

United States

Related Subject Headings

  • Zebrafish
  • Optical Imaging
  • Mycobacterium chelonae
  • Mycobacterium Infections, Nontuberculous
  • Larva
  • Fluorescence
  • Fish Diseases
  • Female
  • Developmental Biology
  • Animals