Vaccine-induced IgG antibodies to V1V2 regions of multiple HIV-1 subtypes correlate with decreased risk of HIV-1 infection.

Published

Journal Article

In the RV144 HIV-1 vaccine efficacy trial, IgG antibody (Ab) binding levels to variable regions 1 and 2 (V1V2) of the HIV-1 envelope glycoprotein gp120 were an inverse correlate of risk of HIV-1 infection. To determine if V1V2-specific Abs cross-react with V1V2 from different HIV-1 subtypes, if the nature of the V1V2 antigen used to asses cross-reactivity influenced infection risk, and to identify immune assays for upcoming HIV-1 vaccine efficacy trials, new V1V2-scaffold antigens were designed and tested. Protein scaffold antigens carrying the V1V2 regions from HIV-1 subtypes A, B, C, D or CRF01_AE were assayed in pilot studies, and six were selected to assess cross-reactive Abs in the plasma from the original RV144 case-control cohort (41 infected vaccinees, 205 frequency-matched uninfected vaccinees, and 40 placebo recipients) using ELISA and a binding Ab multiplex assay. IgG levels to these antigens were assessed as correlates of risk in vaccine recipients using weighted logistic regression models. Levels of Abs reactive with subtype A, B, C and CRF01_AE V1V2-scaffold antigens were all significant inverse correlates of risk (p-values of 0.0008-0.05; estimated odds ratios of 0.53-0.68 per 1 standard deviation increase). Thus, levels of vaccine-induced IgG Abs recognizing V1V2 regions from multiple HIV-1 subtypes, and presented on different scaffolds, constitute inverse correlates of risk for HIV-1 infection in the RV144 vaccine trial. The V1V2 antigens provide a link between RV144 and upcoming HIV-1 vaccine trials, and identify reagents and methods for evaluating V1V2 Abs as possible correlates of protection against HIV-1 infection.ClinicalTrials.gov NCT00223080.

Full Text

Duke Authors

Cited Authors

  • Zolla-Pazner, S; deCamp, A; Gilbert, PB; Williams, C; Yates, NL; Williams, WT; Howington, R; Fong, Y; Morris, DE; Soderberg, KA; Irene, C; Reichman, C; Pinter, A; Parks, R; Pitisuttithum, P; Kaewkungwal, J; Rerks-Ngarm, S; Nitayaphan, S; Andrews, C; O'Connell, RJ; Yang, Z-Y; Nabel, GJ; Kim, JH; Michael, NL; Montefiori, DC; Liao, H-X; Haynes, BF; Tomaras, GD

Published Date

  • January 2014

Published In

Volume / Issue

  • 9 / 2

Start / End Page

  • e87572 -

PubMed ID

  • 24504509

Pubmed Central ID

  • 24504509

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0087572

Language

  • eng