Human and mouse skeletal muscle stem cells: convergent and divergent mechanisms of myogenesis.
Published online
Journal Article
Satellite cells are the chief contributor to skeletal muscle growth and regeneration. The study of mouse satellite cells has accelerated in recent years due to technical advancements in the isolation of these cells. The study of human satellite cells has lagged and thus little is known about how the biology of mouse and human satellite cells compare. We developed a flow cytometry-based method to prospectively isolate human skeletal muscle progenitors from the satellite cell pool using positive and negative selection markers. Results show that this pool is enriched in PAX7 expressing cells that possess robust myogenic potential including the ability to give rise to de novo muscle in vivo. We compared mouse and human satellite cells in culture and identify differences in the elaboration of the myogenic genetic program and in the sensitivity of the cells to cytokine stimulation. These results indicate that not all mechanisms regulating mouse satellite cell activation are conserved in human satellite cells and that such differences may impact the clinical translation of therapeutics validated in mouse models. Thus, the findings of this study are relevant to developing therapies to combat muscle disease.
Full Text
Duke Authors
Cited Authors
- Bareja, A; Holt, JA; Luo, G; Chang, C; Lin, J; Hinken, AC; Freudenberg, JM; Kraus, WE; Evans, WJ; Billin, AN
Published Date
- 2014
Published In
Volume / Issue
- 9 / 2
Start / End Page
- e90398 -
PubMed ID
- 24587351
Pubmed Central ID
- 24587351
Electronic International Standard Serial Number (EISSN)
- 1932-6203
Digital Object Identifier (DOI)
- 10.1371/journal.pone.0090398
Language
- eng
Conference Location
- United States