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Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip.

Publication ,  Journal Article
Ngo, HT; Wang, H-N; Burke, T; Ginsburg, GS; Vo-Dinh, T
Published in: Anal Bioanal Chem
May 2014

Developing techniques for multiplex detection of disease biomarkers is important for clinical diagnosis. In this work, we have demonstrated for the first time the feasibility of multiplex detection of genetic disease biomarkers using the surface-enhanced Raman scattering (SERS)-based molecular sentinel-on-chip (MSC) diagnostic technology. The molecular sentinel (MS) sensing mechanism is based upon the decrease of SERS intensity when Raman labels tagged at 3'-ends of MS nanoprobes are physically displaced from the nanowave chip's surface upon DNA hybridization. The use of bimetallic layer (silver and gold) for the nanowave fabrication was investigated. SERS measurements were performed immediately following a single hybridization reaction between the target single-stranded DNA sequences and the complementary MS nanoprobes immobilized on the nanowave chip without requiring target labeling (i.e., label-free), secondary hybridization, or post-hybridization washing, thus shortening the assay time and reducing cost. Two nucleic acid transcripts, interferon alpha-inducible protein 27 and interferon-induced protein 44-like, are used as model systems for the multiplex detection concept demonstration. These two genes are well known for their critical role in host immune response to viral infection and can be used as molecular signature for viral infection diagnosis. The results indicate the potential of the MSC technology for nucleic acid biomarker multiplex detection.

Duke Scholars

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Published In

Anal Bioanal Chem

DOI

EISSN

1618-2650

Publication Date

May 2014

Volume

406

Issue

14

Start / End Page

3335 / 3344

Location

Germany

Related Subject Headings

  • Time Factors
  • Spectrum Analysis, Raman
  • Silver
  • Oligonucleotides
  • Nucleic Acid Hybridization
  • Nanotechnology
  • Microscopy, Electron, Scanning
  • Membrane Proteins
  • Mass Spectrometry
  • Immune System
 

Citation

APA
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ICMJE
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Ngo, H. T., Wang, H.-N., Burke, T., Ginsburg, G. S., & Vo-Dinh, T. (2014). Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip. Anal Bioanal Chem, 406(14), 3335–3344. https://doi.org/10.1007/s00216-014-7648-4
Ngo, Hoan T., Hsin-Neng Wang, Thomas Burke, Geoffrey S. Ginsburg, and Tuan Vo-Dinh. “Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip.Anal Bioanal Chem 406, no. 14 (May 2014): 3335–44. https://doi.org/10.1007/s00216-014-7648-4.
Ngo HT, Wang H-N, Burke T, Ginsburg GS, Vo-Dinh T. Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip. Anal Bioanal Chem. 2014 May;406(14):3335–44.
Ngo, Hoan T., et al. “Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip.Anal Bioanal Chem, vol. 406, no. 14, May 2014, pp. 3335–44. Pubmed, doi:10.1007/s00216-014-7648-4.
Ngo HT, Wang H-N, Burke T, Ginsburg GS, Vo-Dinh T. Multiplex detection of disease biomarkers using SERS molecular sentinel-on-chip. Anal Bioanal Chem. 2014 May;406(14):3335–3344.
Journal cover image

Published In

Anal Bioanal Chem

DOI

EISSN

1618-2650

Publication Date

May 2014

Volume

406

Issue

14

Start / End Page

3335 / 3344

Location

Germany

Related Subject Headings

  • Time Factors
  • Spectrum Analysis, Raman
  • Silver
  • Oligonucleotides
  • Nucleic Acid Hybridization
  • Nanotechnology
  • Microscopy, Electron, Scanning
  • Membrane Proteins
  • Mass Spectrometry
  • Immune System