Perturbations to the hedgehog pathway in sea urchin embryos.
Journal Article (Journal Article)
The Hedgehog pathway has been shown to be an important developmental signaling pathway in many organisms (Ingham and McMahon. Genes Dev 15:3059-3087, 2001). Recently that work has been extended to developing echinoderm embryos (Walton et al. Dev Biol 331(1):26-37, 2009). Here we describe several methods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.
Full Text
Duke Authors
Cited Authors
- Warner, JF; McClay, DR
Published Date
- January 2014
Published In
Volume / Issue
- 1128 /
Start / End Page
- 211 - 221
PubMed ID
- 24567217
Electronic International Standard Serial Number (EISSN)
- 1940-6029
International Standard Serial Number (ISSN)
- 1064-3745
Digital Object Identifier (DOI)
- 10.1007/978-1-62703-974-1_14
Language
- eng