Perturbations to the hedgehog pathway in sea urchin embryos.

Journal Article (Journal Article)

The Hedgehog pathway has been shown to be an important developmental signaling pathway in many organisms (Ingham and McMahon. Genes Dev 15:3059-3087, 2001). Recently that work has been extended to developing echinoderm embryos (Walton et al. Dev Biol 331(1):26-37, 2009). Here we describe several methods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.

Full Text

Duke Authors

Cited Authors

  • Warner, JF; McClay, DR

Published Date

  • January 2014

Published In

Volume / Issue

  • 1128 /

Start / End Page

  • 211 - 221

PubMed ID

  • 24567217

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-974-1_14


  • eng