Perturbations to the hedgehog pathway in sea urchin embryos.
Publication
, Journal Article
Warner, JF; McClay, DR
Published in: Methods in molecular biology (Clifton, N.J.)
January 2014
The Hedgehog pathway has been shown to be an important developmental signaling pathway in many organisms (Ingham and McMahon. Genes Dev 15:3059-3087, 2001). Recently that work has been extended to developing echinoderm embryos (Walton et al. Dev Biol 331(1):26-37, 2009). Here we describe several methods to perturb the Hedgehog signaling pathway in the sea urchin. These include microinjection of Morpholinos and mRNA constructs as well as treatments with small molecule inhibitors. Finally we provide simple methods for assaying Hedgehog phenotypes in the sea urchin embryo.
Duke Scholars
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Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1128
Start / End Page
211 / 221
Related Subject Headings
- Tissue Culture Techniques
- Signal Transduction
- Sea Urchins
- RNA, Messenger
- Morpholinos
- Microinjections
- Hedgehog Proteins
- Gene Knockdown Techniques
- Embryo, Nonmammalian
- Developmental Biology
Citation
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Warner, J. F., & McClay, D. R. (2014). Perturbations to the hedgehog pathway in sea urchin embryos. Methods in Molecular Biology (Clifton, N.J.), 1128, 211–221. https://doi.org/10.1007/978-1-62703-974-1_14
Warner, Jacob F., and David R. McClay. “Perturbations to the hedgehog pathway in sea urchin embryos.” Methods in Molecular Biology (Clifton, N.J.) 1128 (January 2014): 211–21. https://doi.org/10.1007/978-1-62703-974-1_14.
Warner JF, McClay DR. Perturbations to the hedgehog pathway in sea urchin embryos. Methods in molecular biology (Clifton, NJ). 2014 Jan;1128:211–21.
Warner, Jacob F., and David R. McClay. “Perturbations to the hedgehog pathway in sea urchin embryos.” Methods in Molecular Biology (Clifton, N.J.), vol. 1128, Jan. 2014, pp. 211–21. Epmc, doi:10.1007/978-1-62703-974-1_14.
Warner JF, McClay DR. Perturbations to the hedgehog pathway in sea urchin embryos. Methods in molecular biology (Clifton, NJ). 2014 Jan;1128:211–221.
Published In
Methods in molecular biology (Clifton, N.J.)
DOI
EISSN
1940-6029
ISSN
1064-3745
Publication Date
January 2014
Volume
1128
Start / End Page
211 / 221
Related Subject Headings
- Tissue Culture Techniques
- Signal Transduction
- Sea Urchins
- RNA, Messenger
- Morpholinos
- Microinjections
- Hedgehog Proteins
- Gene Knockdown Techniques
- Embryo, Nonmammalian
- Developmental Biology