Skip to main content

The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers.

Publication ,  Journal Article
Mobarrez, F; Antoniewicz, L; Bosson, JA; Kuhl, J; Pisetsky, DS; Lundbäck, M
Published in: PLoS One
2014

BACKGROUND: Cigarette smoking, both active and passive, is one of the leading causes of morbidity and mortality in cardiovascular disease. To assess the impact of brief smoking on the vasculature, we determined levels of circulating endothelial progenitor cells (EPCs) and circulating microparticles (MPs) following the smoking of one cigarette by young, healthy intermittent smokers. MATERIALS AND METHODS: 12 healthy volunteers were randomized to either smoking or not smoking in a crossover fashion. Blood sampling was performed at baseline, 1, 4 and 24 hours following smoking/not smoking. The numbers of EPCs and MPs were determined by flow cytometry. MPs were measured from platelets, leukocytes and endothelial cells. Moreover, MPs were also labelled with anti-HMGB1 and SYTO 13 to assess the content of nuclear molecules. RESULTS: Active smoking of one cigarette caused an immediate and significant increase in the numbers of circulating EPCs and MPs of platelet-, endothelial- and leukocyte origin. Levels of MPs containing nuclear molecules were increased, of which the majority were positive for CD41 and CD45 (platelet- and leukocyte origin). CD144 (VE-cadherin) or HMGB1 release did not significantly change during active smoking. CONCLUSION: Brief active smoking of one cigarette generated an acute release of EPC and MPs, of which the latter contained nuclear matter. Together, these results demonstrate acute effects of cigarette smoke on endothelial, platelet and leukocyte function as well as injury to the vascular wall.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

2

Start / End Page

e90314

Location

United States

Related Subject Headings

  • Stem Cells
  • Smoking
  • Platelet Membrane Glycoprotein IIb
  • Male
  • Leukocytes
  • Leukocyte Common Antigens
  • Humans
  • Healthy Volunteers
  • HMGB1 Protein
  • General Science & Technology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mobarrez, F., Antoniewicz, L., Bosson, J. A., Kuhl, J., Pisetsky, D. S., & Lundbäck, M. (2014). The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers. PLoS One, 9(2), e90314. https://doi.org/10.1371/journal.pone.0090314
Mobarrez, Fariborz, Lukasz Antoniewicz, Jenny A. Bosson, Jeanette Kuhl, David S. Pisetsky, and Magnus Lundbäck. “The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers.PLoS One 9, no. 2 (2014): e90314. https://doi.org/10.1371/journal.pone.0090314.
Mobarrez F, Antoniewicz L, Bosson JA, Kuhl J, Pisetsky DS, Lundbäck M. The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers. PLoS One. 2014;9(2):e90314.
Mobarrez, Fariborz, et al. “The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers.PLoS One, vol. 9, no. 2, 2014, p. e90314. Pubmed, doi:10.1371/journal.pone.0090314.
Mobarrez F, Antoniewicz L, Bosson JA, Kuhl J, Pisetsky DS, Lundbäck M. The effects of smoking on levels of endothelial progenitor cells and microparticles in the blood of healthy volunteers. PLoS One. 2014;9(2):e90314.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2014

Volume

9

Issue

2

Start / End Page

e90314

Location

United States

Related Subject Headings

  • Stem Cells
  • Smoking
  • Platelet Membrane Glycoprotein IIb
  • Male
  • Leukocytes
  • Leukocyte Common Antigens
  • Humans
  • Healthy Volunteers
  • HMGB1 Protein
  • General Science & Technology