Mobile elements and mitochondrial genome expansion in the soil fungus and potato pathogen Rhizoctonia solani AG-3.

Journal Article (Letter)

The soil fungus Rhizoctonia solani is an economically important pathogen of agricultural and forestry crops. Here, we present the complete sequence and analysis of the mitochondrial genome of R. solani, field isolate Rhs1AP. The genome (235 849 bp) is the largest mitochondrial genome of a filamentous fungus sequenced to date and exhibits a rich accumulation of introns, novel repeat sequences, homing endonuclease genes, and hypothetical genes. Stable secondary structures exhibited by repeat sequences suggest that they comprise functional, possibly catalytic RNA elements. RNA-Seq expression profiling confirmed that the majority of homing endonuclease genes and hypothetical genes are transcriptionally active. Comparative analysis suggests that the mitochondrial genome of R. solani is an example of a dynamic history of expansion in filamentous fungi.

Full Text

Duke Authors

Cited Authors

  • Losada, L; Pakala, SB; Fedorova, ND; Joardar, V; Shabalina, SA; Hostetler, J; Pakala, SM; Zafar, N; Thomas, E; Rodriguez-Carres, M; Dean, R; Vilgalys, R; Nierman, WC; Cubeta, MA

Published Date

  • March 2014

Published In

Volume / Issue

  • 352 / 2

Start / End Page

  • 165 - 173

PubMed ID

  • 24461055

Electronic International Standard Serial Number (EISSN)

  • 1574-6968

International Standard Serial Number (ISSN)

  • 0378-1097

Digital Object Identifier (DOI)

  • 10.1111/1574-6968.12387

Language

  • eng