Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.

Published

Journal Article

Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury. Here, we describe a platform for engineering and testing highly functional biomimetic muscle tissues with a resident satellite cell niche and capacity for robust myogenesis and self-regeneration in vitro. Using a mouse dorsal window implantation model and transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively monitored, in real time, vascular integration and the functional state of engineered muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady ingrowth of blood-perfused microvasculature along with an increase in amplitude of calcium transients and force of contraction. We also demonstrated superior structural organization, vascularization, and contractile function of fully differentiated vs. undifferentiated engineered muscle implants. The described in vitro and in vivo models of biomimetic engineered muscle represent enabling technology for novel studies of skeletal muscle function and regeneration.

Full Text

Duke Authors

Cited Authors

  • Juhas, M; Engelmayr, GC; Fontanella, AN; Palmer, GM; Bursac, N

Published Date

  • April 15, 2014

Published In

Volume / Issue

  • 111 / 15

Start / End Page

  • 5508 - 5513

PubMed ID

  • 24706792

Pubmed Central ID

  • 24706792

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1402723111

Language

  • eng

Conference Location

  • United States