Identification of early interactions between Francisella and the host.

Published

Journal Article

The adaptive immune response to Francisella tularensis is dependent on the route of inoculation. Intradermal inoculation with the F. tularensis live vaccine strain (LVS) results in a robust Th1 response in the lungs, whereas intranasal inoculation produces fewer Th1 cells and instead many Th17 cells. Interestingly, bacterial loads in the lungs are similar early after inoculation by these two routes. We hypothesize that the adaptive immune response is influenced by local events in the lungs, such as the type of cells that are first infected with Francisella. Using fluorescence-activated cell sorting, we identified alveolar macrophages as the first cell type infected in the lungs of mice intranasally inoculated with F. novicida U112, LVS, or F. tularensis Schu S4. Following bacterial dissemination from the skin to the lung, interstitial macrophages or neutrophils are infected. Overall, we identified the early interactions between Francisella and the host following two different routes of inoculation.

Full Text

Duke Authors

Cited Authors

  • Roberts, LM; Tuladhar, S; Steele, SP; Riebe, KJ; Chen, C-J; Cumming, RI; Seay, S; Frothingham, R; Sempowski, GD; Kawula, TH; Frelinger, JA

Published Date

  • June 2014

Published In

Volume / Issue

  • 82 / 6

Start / End Page

  • 2504 - 2510

PubMed ID

  • 24686053

Pubmed Central ID

  • 24686053

Electronic International Standard Serial Number (EISSN)

  • 1098-5522

International Standard Serial Number (ISSN)

  • 0019-9567

Digital Object Identifier (DOI)

  • 10.1128/IAI.01654-13

Language

  • eng