Identification of early interactions between Francisella and the host.
Journal Article (Journal Article)
The adaptive immune response to Francisella tularensis is dependent on the route of inoculation. Intradermal inoculation with the F. tularensis live vaccine strain (LVS) results in a robust Th1 response in the lungs, whereas intranasal inoculation produces fewer Th1 cells and instead many Th17 cells. Interestingly, bacterial loads in the lungs are similar early after inoculation by these two routes. We hypothesize that the adaptive immune response is influenced by local events in the lungs, such as the type of cells that are first infected with Francisella. Using fluorescence-activated cell sorting, we identified alveolar macrophages as the first cell type infected in the lungs of mice intranasally inoculated with F. novicida U112, LVS, or F. tularensis Schu S4. Following bacterial dissemination from the skin to the lung, interstitial macrophages or neutrophils are infected. Overall, we identified the early interactions between Francisella and the host following two different routes of inoculation.
Full Text
Duke Authors
Cited Authors
- Roberts, LM; Tuladhar, S; Steele, SP; Riebe, KJ; Chen, C-J; Cumming, RI; Seay, S; Frothingham, R; Sempowski, GD; Kawula, TH; Frelinger, JA
Published Date
- June 2014
Published In
Volume / Issue
- 82 / 6
Start / End Page
- 2504 - 2510
PubMed ID
- 24686053
Pubmed Central ID
- PMC4019147
Electronic International Standard Serial Number (EISSN)
- 1098-5522
Digital Object Identifier (DOI)
- 10.1128/IAI.01654-13
Language
- eng
Conference Location
- United States