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ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo.

Publication ,  Journal Article
Hagedorn, EJ; Kelley, LC; Naegeli, KM; Wang, Z; Chi, Q; Sherwood, DR
Published in: The Journal of cell biology
March 2014

Invadopodia are protrusive, F-actin-driven membrane structures that are thought to mediate basement membrane transmigration during development and tumor dissemination. An understanding of the mechanisms regulating invadopodia has been hindered by the difficulty of examining these dynamic structures in native environments. Using an RNAi screen and live-cell imaging of anchor cell (AC) invasion in Caenorhabditis elegans, we have identified UNC-60A (ADF/cofilin) as an essential regulator of invadopodia. UNC-60A localizes to AC invadopodia, and its loss resulted in a dramatic slowing of F-actin dynamics and an inability to breach basement membrane. Optical highlighting indicated that UNC-60A disassembles actin filaments at invadopodia. Surprisingly, loss of unc-60a led to the accumulation of invadopodial membrane and associated components within the endolysosomal compartment. Photobleaching experiments revealed that during normal invasion the invadopodial membrane undergoes rapid recycling through the endolysosome. Together, these results identify the invadopodial membrane as a specialized compartment whose recycling to form dynamic, functional invadopodia is dependent on localized F-actin disassembly by ADF/cofilin.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

March 2014

Volume

204

Issue

7

Start / End Page

1209 / 1218

Related Subject Headings

  • Time-Lapse Imaging
  • Protein Transport
  • Protein Multimerization
  • Microscopy, Fluorescence
  • Microfilament Proteins
  • Lysosomes
  • Endosomes
  • Developmental Biology
  • Cell Surface Extensions
  • Cell Polarity
 

Citation

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Hagedorn, E. J., Kelley, L. C., Naegeli, K. M., Wang, Z., Chi, Q., & Sherwood, D. R. (2014). ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo. The Journal of Cell Biology, 204(7), 1209–1218. https://doi.org/10.1083/jcb.201312098
Hagedorn, Elliott J., Laura C. Kelley, Kaleb M. Naegeli, Zheng Wang, Qiuyi Chi, and David R. Sherwood. “ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo.The Journal of Cell Biology 204, no. 7 (March 2014): 1209–18. https://doi.org/10.1083/jcb.201312098.
Hagedorn EJ, Kelley LC, Naegeli KM, Wang Z, Chi Q, Sherwood DR. ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo. The Journal of cell biology. 2014 Mar;204(7):1209–18.
Hagedorn, Elliott J., et al. “ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo.The Journal of Cell Biology, vol. 204, no. 7, Mar. 2014, pp. 1209–18. Epmc, doi:10.1083/jcb.201312098.
Hagedorn EJ, Kelley LC, Naegeli KM, Wang Z, Chi Q, Sherwood DR. ADF/cofilin promotes invadopodial membrane recycling during cell invasion in vivo. The Journal of cell biology. 2014 Mar;204(7):1209–1218.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

March 2014

Volume

204

Issue

7

Start / End Page

1209 / 1218

Related Subject Headings

  • Time-Lapse Imaging
  • Protein Transport
  • Protein Multimerization
  • Microscopy, Fluorescence
  • Microfilament Proteins
  • Lysosomes
  • Endosomes
  • Developmental Biology
  • Cell Surface Extensions
  • Cell Polarity