Synthesis of fluorosurfactants for emulsion-based biological applications.


Journal Article

Microemulsion represents an attractive platform for fundamental and applied biomedical research because the emulsified droplets can serve as millions of compartmentalized micrometer-sized reactors amenable to high-throughput screening or online monitoring. However, establishing stable emulsions with surfactants that are compatible with biological applications remains a significant challenge. Motivated by the lack of commercially available surfactants suitable for microemulsion-based biological assays, this study describes the facile synthesis of a biocompatible fluorosurfactant with nonionic tris(hydroxymethyl)methyl (Tris) polar head groups. We have further demonstrated compatibility of the developed surfactant with diverse emulsion-based applications, including DNA polymeric nanoparticle synthesis, enzymatic activity assay, and bacterial or mammalian cell culture, in the setup of both double- and multiphases of emulsions.

Full Text

Cited Authors

  • Chiu, Y-L; Chan, HF; Phua, KKL; Zhang, Y; Juul, S; Knudsen, BR; Ho, Y-P; Leong, KW

Published Date

  • April 2014

Published In

Volume / Issue

  • 8 / 4

Start / End Page

  • 3913 - 3920

PubMed ID

  • 24646088

Pubmed Central ID

  • 24646088

Electronic International Standard Serial Number (EISSN)

  • 1936-086X

International Standard Serial Number (ISSN)

  • 1936-0851

Digital Object Identifier (DOI)

  • 10.1021/nn500810n


  • eng