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Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats.

Publication ,  Journal Article
Liu, M; Shen, L; Begg, DP; D'alessio, DA; Woods, SC
Published in: J Neurosci Methods
July 30, 2012

Cerebrospinal fluid (CSF) provides an invaluable analytical window to the central nervous system (CNS) because it reflects the dynamically changing complement of CNS constituents. We describe an improved method for sampling CSF in rats that is easy to perform. It has a 96% success rate of CSF collection and consistently yields large volumes (150-200 μl) of CSF. The blood contamination rate is also low (6%) as determined by both visual inspection and the lack of molecular detection of apolipoprotein B, a plasma-derived protein, which is absent in the CNS. This improved method of CSF sampling can have broad applicability in physiological and pharmacological evaluation for diverse CNS targets. We used this technique to provide proof of principle by examining the effect of intraperitoneal insulin on the level of apolipoprotein E (apoE) in the CSF. Insulin (0.5 and 1 U/kg) led to a significant increase of insulin in both plasma and CSF at 2 h after intraperitoneal administration and decreased blood glucose for at least 2h. ApoE concentrations in CSF, but not in plasma, were also significantly increased, and its time-course was inversely correlated with the alterations in blood glucose over 2 h. These results provide a pharmacological validation of the novel CSF sampling and validation procedure for sampling rat CSF.

Duke Scholars

Published In

J Neurosci Methods

DOI

EISSN

1872-678X

Publication Date

July 30, 2012

Volume

209

Issue

1

Start / End Page

106 / 112

Location

Netherlands

Related Subject Headings

  • Rats, Long-Evans
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Insulin
  • Cerebrospinal Fluid
  • Blotting, Western
  • Apolipoproteins E
  • Animals
  • 3209 Neurosciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liu, M., Shen, L., Begg, D. P., D’alessio, D. A., & Woods, S. C. (2012). Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats. J Neurosci Methods, 209(1), 106–112. https://doi.org/10.1016/j.jneumeth.2012.05.034
Liu, Min, Ling Shen, Denovan P. Begg, David A. D’alessio, and Stephen C. Woods. “Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats.J Neurosci Methods 209, no. 1 (July 30, 2012): 106–12. https://doi.org/10.1016/j.jneumeth.2012.05.034.
Liu M, Shen L, Begg DP, D’alessio DA, Woods SC. Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats. J Neurosci Methods. 2012 Jul 30;209(1):106–12.
Liu, Min, et al. “Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats.J Neurosci Methods, vol. 209, no. 1, July 2012, pp. 106–12. Pubmed, doi:10.1016/j.jneumeth.2012.05.034.
Liu M, Shen L, Begg DP, D’alessio DA, Woods SC. Insulin increases central apolipoprotein E levels as revealed by an improved technique for collection of cerebrospinal fluid from rats. J Neurosci Methods. 2012 Jul 30;209(1):106–112.
Journal cover image

Published In

J Neurosci Methods

DOI

EISSN

1872-678X

Publication Date

July 30, 2012

Volume

209

Issue

1

Start / End Page

106 / 112

Location

Netherlands

Related Subject Headings

  • Rats, Long-Evans
  • Rats
  • Neurology & Neurosurgery
  • Male
  • Insulin
  • Cerebrospinal Fluid
  • Blotting, Western
  • Apolipoproteins E
  • Animals
  • 3209 Neurosciences