Insulin receptor substrate-1 (IRS-1) expression in rat brain.


Journal Article

IRS-1 is phosphorylated on tyrosine residues after insulin stimulation and participates in the early events of signal transduction in peripheral insulin-sensitive tissues. This study determined whether neuronal populations in the rat olfactory bulb and hippocampus (brain regions which have very high concentrations of insulin receptors) also express IRS-1 and contain phosphotyrosine, using in situ hybridization, receptor binding, and immunocytochemistry. IRS-1 mRNA was colocalized with insulin receptor mRNA in neuron cell bodies of hippocampus and olfactory bulb. Similarly, IRS-1 immunoreactivity in hippocampus and olfactory bulb was concentrated in layers that contain synapses of these neurons and have both high insulin binding and phosphotyrosine levels. Thus, IRS-1 and insulin receptors are coexpressed in discrete populations of neurons, suggesting a signal transduction mechanism by which insulin may influence metabolism and gene expression in the brain.

Full Text

Duke Authors

Cited Authors

  • Baskin, DG; Schwartz, MW; Sipols, AJ; D'Alessio, DA; Goldstein, BJ; White, MF

Published Date

  • April 1, 1994

Published In

Volume / Issue

  • 134 / 4

Start / End Page

  • 1952 - 1955

PubMed ID

  • 7511094

Pubmed Central ID

  • 7511094

International Standard Serial Number (ISSN)

  • 0013-7227

Digital Object Identifier (DOI)

  • 10.1210/endo.134.4.7511094


  • eng

Conference Location

  • United States