The effect of polyamines on the binding of anti-DNA antibodies from patients with SLE and normal human subjects.

Published

Journal Article

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE). To elucidate specificity further, the effect of polyamines on the binding of anti-DNA antibodies from patients with lupus was tested by ELISA to calf thymus (CT) DNA; we also assessed the binding of plasmas of patients and normal human subjects (NHS) to Micrococcus luteus (MC) DNA. As these studies showed, spermine can dose-dependently inhibit SLE anti-DNA binding to CT DNA and can promote dissociation of preformed immune complexes. With MC DNA as antigen, spermine failed to inhibit the NHS anti-DNA binding. Studies using plasmas adsorbed to a CT DNA cellulose affinity indicated that SLE plasmas are mixtures of anti-DNA that differ in inhibition by spermine and binding to conserved and non-conserved determinants. Together, these studies demonstrate that spermine can influence the binding of anti-DNA autoantibodies and may contribute to the antigenicity of DNA.

Full Text

Duke Authors

Cited Authors

  • Wang, X; Stearns, NA; Li, X; Pisetsky, DS

Published Date

  • July 2014

Published In

Volume / Issue

  • 153 / 1

Start / End Page

  • 94 - 103

PubMed ID

  • 24732074

Pubmed Central ID

  • 24732074

Electronic International Standard Serial Number (EISSN)

  • 1521-7035

Digital Object Identifier (DOI)

  • 10.1016/j.clim.2014.04.003

Language

  • eng

Conference Location

  • United States