WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.

Journal Article (Journal Article)

Wnts are transported to the cell surface by the integral membrane protein WLS (also known as Wntless, Evi, and GPR177). Previous studies of WLS trafficking have emphasized WLS movement from the Golgi to the plasma membrane (PM) and then back to the Golgi via retromer-mediated endocytic recycling. We find that endogenous WLS binds Wnts in the endoplasmic reticulum (ER), cycles to the PM, and then returns to the ER through the Golgi. We identify an ER-targeting sequence at the carboxyl terminus of native WLS that is critical for ER retrograde recycling and contributes to Wnt secretory function. Golgi-to-ER recycling of WLS requires the COPI regulator ARF as well as ERGIC2, an ER-Golgi intermediate compartment protein that is also required for the retrograde trafficking of the KDEL receptor and certain toxins. ERGIC2 is required for efficient Wnt secretion. ER retrieval is an integral part of the WLS transport cycle.

Full Text

Duke Authors

Cited Authors

  • Yu, J; Chia, J; Canning, CA; Jones, CM; Bard, FA; Virshup, DM

Published Date

  • May 12, 2014

Published In

Volume / Issue

  • 29 / 3

Start / End Page

  • 277 - 291

PubMed ID

  • 24768165

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2014.03.016


  • eng

Conference Location

  • United States