Controlling the structural and functional anisotropy of engineered cardiac tissues.

Published

Journal Article

The ability to control the degree of structural and functional anisotropy in 3D engineered cardiac tissues would have high utility for both in vitro studies of cardiac muscle physiology and pathology as well as potential tissue engineering therapies for myocardial infarction. Here, we applied a high aspect ratio soft lithography technique to generate network-like tissue patches seeded with neonatal rat cardiomyocytes. Fabricating longer elliptical pores within the patch networks increased the overall cardiomyocyte and extracellular matrix alignment within the patch. Improved uniformity of cell and matrix alignment yielded an increase in anisotropy of action potential propagation and faster longitudinal conduction velocity (LCV). Cardiac tissue patches with a higher degree of cardiomyocyte alignment and electrical anisotropy also demonstrated greater isometric twitch forces. After two weeks of culture, specific measures of electrical and contractile function (LCV = 26.8 ± 0.8 cm s(-1), specific twitch force = 8.9 ± 1.1 mN mm(-2) for the longest pores studied) were comparable to those of neonatal rat myocardium. We have thus described methodology for engineering of highly functional 3D engineered cardiac tissues with controllable degree of anisotropy.

Full Text

Duke Authors

Cited Authors

  • Bian, W; Jackman, CP; Bursac, N

Published Date

  • June 2014

Published In

Volume / Issue

  • 6 / 2

Start / End Page

  • 24109 - 24109

PubMed ID

  • 24717534

Pubmed Central ID

  • 24717534

Electronic International Standard Serial Number (EISSN)

  • 1758-5090

International Standard Serial Number (ISSN)

  • 1758-5082

Digital Object Identifier (DOI)

  • 10.1088/1758-5082/6/2/024109

Language

  • eng