Skip to main content
Journal cover image

The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies.

Publication ,  Journal Article
Wong, WW; Roberts, SB; Racette, SB; Das, SK; Redman, LM; Rochon, J; Bhapkar, MV; Clarke, LL; Kraus, WE
Published in: J Nutr
May 2014

The doubly labeled water (DLW) method is considered the reference method for the measurement of energy expenditure under free-living conditions. However, the reproducibility of the DLW method in longitudinal studies is not well documented. This study was designed to evaluate the longitudinal reproducibility of the DLW method using 2 protocols developed and implemented in a multicenter clinical trial-the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE). To document the longitudinal reproducibility of the DLW method, 2 protocols, 1 based on repeated analysis of dose dilutions over the course of the clinical trial (dose-dilution protocol) and 1 based on repeated but blinded analysis of randomly selected DLW studies (test-retest protocol), were carried out. The dose-dilution protocol showed that the theoretical fractional turnover rates for (2)H and (18)O and the difference between the 2 fractional turnover rates were reproducible to within 1% and 5%, respectively, over 4.5 y. The Bland-Altman pair-wise comparisons of the results generated from 50 test-retest DLW studies showed that the fractional turnover rates and isotope dilution spaces for (2)H and (18)O, and total energy expenditure, were highly reproducible over 2.4 y. Our results show that the DLW method is reproducible in longitudinal studies and confirm the validity of this method to measure energy expenditure, define energy intake prescriptions, and monitor adherence and body composition changes over the period of 2.5-4.4 y. The 2 protocols can be adopted by other laboratories to document the longitudinal reproducibility of their measurements to ensure the long-term outcomes of interest are meaningful biologically. This trial was registered at clinicaltrials.gov as NCT00427193.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Nutr

DOI

EISSN

1541-6100

Publication Date

May 2014

Volume

144

Issue

5

Start / End Page

777 / 783

Location

United States

Related Subject Headings

  • Young Adult
  • Water
  • Reproducibility of Results
  • Oxygen Isotopes
  • Nutrition & Dietetics
  • Models, Biological
  • Middle Aged
  • Male
  • Longitudinal Studies
  • Isotope Labeling
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wong, W. W., Roberts, S. B., Racette, S. B., Das, S. K., Redman, L. M., Rochon, J., … Kraus, W. E. (2014). The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies. J Nutr, 144(5), 777–783. https://doi.org/10.3945/jn.113.187823
Wong, William W., Susan B. Roberts, Susan B. Racette, Sai Krupa Das, Leanne M. Redman, James Rochon, Manjushri V. Bhapkar, Lucinda L. Clarke, and William E. Kraus. “The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies.J Nutr 144, no. 5 (May 2014): 777–83. https://doi.org/10.3945/jn.113.187823.
Wong WW, Roberts SB, Racette SB, Das SK, Redman LM, Rochon J, et al. The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies. J Nutr. 2014 May;144(5):777–83.
Wong, William W., et al. “The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies.J Nutr, vol. 144, no. 5, May 2014, pp. 777–83. Pubmed, doi:10.3945/jn.113.187823.
Wong WW, Roberts SB, Racette SB, Das SK, Redman LM, Rochon J, Bhapkar MV, Clarke LL, Kraus WE. The doubly labeled water method produces highly reproducible longitudinal results in nutrition studies. J Nutr. 2014 May;144(5):777–783.
Journal cover image

Published In

J Nutr

DOI

EISSN

1541-6100

Publication Date

May 2014

Volume

144

Issue

5

Start / End Page

777 / 783

Location

United States

Related Subject Headings

  • Young Adult
  • Water
  • Reproducibility of Results
  • Oxygen Isotopes
  • Nutrition & Dietetics
  • Models, Biological
  • Middle Aged
  • Male
  • Longitudinal Studies
  • Isotope Labeling