Sequencing and characterization of oligosaccharides using infrared multiphoton dissociation and boronic acid derivatization in a quadrupole ion trap.

Journal Article (Journal Article)

A simplified method for determining the sequence and branching of oligosaccharides using infrared multiphoton dissociation (IRMPD) in a quadrupole ion trap (QIT) is described. An IR-active boronic acid (IRABA) reagent is used to derivatize the oligosaccharides before IRMPD analysis. The IRABA ligand is designed to both enhance the efficiency of the derivatization reaction and to facilitate the photon absorption process. The resulting IRMPD spectra display oligosaccharide fragments that are formed from primarily one type of diagnostic cleavage, thus making sequencing straightforward. The presence of sequential fragment ions, a phenomenon of IRMPD, permit the comprehensive sequencing of the oligosaccharides studied in a single stage of activation. We demonstrate this approach for two series of oligosaccharides, the lacto-N-fucopentaoses (LNFPs) and the lacto-N-difucohexaoses (LNDFHs).

Full Text

Duke Authors

Cited Authors

  • Pikulski, M; Hargrove, A; Shabbir, SH; Anslyn, EV; Brodbelt, JS

Published Date

  • December 2007

Published In

Volume / Issue

  • 18 / 12

Start / End Page

  • 2094 - 2106

PubMed ID

  • 17936010

Electronic International Standard Serial Number (EISSN)

  • 1879-1123

International Standard Serial Number (ISSN)

  • 1044-0305

Digital Object Identifier (DOI)

  • 10.1016/j.jasms.2007.09.005


  • eng