Skip to main content

Demineralized murine skeletal histology.

Publication ,  Journal Article
Mack, SA; Maltby, KM; Hilton, MJ
Published in: Methods Mol Biol
2014

Cartilage and bone are specialized skeletal tissues composed of unique extracellular matrices. Bone, in particular, has a highly calcified or mineralized matrix that makes microtomy and standard histological studies very challenging. Therefore, methods to appropriately fix and decalcify mineralized skeletal tissues have been developed to allow for paraffin processing and standard microtomy. In this chapter, we illustrate methods for tissue grossing, fixation, decalcification, paraffin processing, embedding, sectioning, and routine histological staining of demineralized murine skeletal tissues. We also discuss methods for decalcified frozen sectioning of skeletal tissues with and without the use of a tape-transfer system.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1130

Start / End Page

165 / 183

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Paraffin Embedding
  • Microtomy
  • Mice
  • Histocytological Preparation Techniques
  • Frozen Sections
  • Developmental Biology
  • Decalcification Technique
  • Bone and Bones
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mack, S. A., Maltby, K. M., & Hilton, M. J. (2014). Demineralized murine skeletal histology. Methods Mol Biol, 1130, 165–183. https://doi.org/10.1007/978-1-62703-989-5_12
Mack, Sarah A., Kathleen M. Maltby, and Matthew J. Hilton. “Demineralized murine skeletal histology.Methods Mol Biol 1130 (2014): 165–83. https://doi.org/10.1007/978-1-62703-989-5_12.
Mack SA, Maltby KM, Hilton MJ. Demineralized murine skeletal histology. Methods Mol Biol. 2014;1130:165–83.
Mack, Sarah A., et al. “Demineralized murine skeletal histology.Methods Mol Biol, vol. 1130, 2014, pp. 165–83. Pubmed, doi:10.1007/978-1-62703-989-5_12.
Mack SA, Maltby KM, Hilton MJ. Demineralized murine skeletal histology. Methods Mol Biol. 2014;1130:165–183.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2014

Volume

1130

Start / End Page

165 / 183

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Paraffin Embedding
  • Microtomy
  • Mice
  • Histocytological Preparation Techniques
  • Frozen Sections
  • Developmental Biology
  • Decalcification Technique
  • Bone and Bones
  • Animals