Whole-mount in situ hybridization on murine skeletogenic tissues.

Journal Article

Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction.

Full Text

Duke Authors

Cited Authors

  • Rutkowsky, T; Sharma, D; Hilton, MJ

Published Date

  • January 2014

Published In

Volume / Issue

  • 1130 /

Start / End Page

  • 193 - 201

PubMed ID

  • 24482174

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-989-5_14

Language

  • eng