Analyzing transcription dynamics during the budding yeast cell cycle.

Journal Article

Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle.

Full Text

Duke Authors

Cited Authors

  • Leman, AR; Bristow, SL; Haase, SB

Published Date

  • January 2014

Published In

Volume / Issue

  • 1170 /

Start / End Page

  • 295 - 312

PubMed ID

  • 24906319

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-0888-2_14

Language

  • eng