Analyzing transcription dynamics during the budding yeast cell cycle.
Journal Article (Journal Article)
Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle.
Full Text
Duke Authors
Cited Authors
- Leman, AR; Bristow, SL; Haase, SB
Published Date
- January 2014
Published In
Volume / Issue
- 1170 /
Start / End Page
- 295 - 312
PubMed ID
- 24906319
Electronic International Standard Serial Number (EISSN)
- 1940-6029
International Standard Serial Number (ISSN)
- 1064-3745
Digital Object Identifier (DOI)
- 10.1007/978-1-4939-0888-2_14
Language
- eng