Humoral immunity induced by mucosal and/or systemic SIV-specific vaccine platforms suggests novel combinatorial approaches for enhancing responses.

Published

Journal Article

Combinatorial HIV/SIV vaccine approaches targeting multiple arms of the immune system might improve protective efficacy. We compared SIV-specific humoral immunity induced in rhesus macaques by five vaccine regimens. Systemic regimens included ALVAC-SIVenv priming and Env boosting (ALVAC/Env); DNA immunization; and DNA plus Env co-immunization (DNA&Env). RepAd/Env combined mucosal replication-competent Ad-env priming with systemic Env boosting. A Peptide/Env regimen, given solely intrarectally, included HIV/SIV peptides followed by MVA-env and Env boosts. Serum antibodies mediating neutralizing, phagocytic and ADCC activities were induced by ALVAC/Env, RepAd/Env and DNA&Env vaccines. Memory B cells and plasma cells were maintained in the bone marrow. RepAd/Env vaccination induced early SIV-specific IgA in rectal secretions before Env boosting, although mucosal IgA and IgG responses were readily detected at necropsy in ALVAC/Env, RepAd/Env, DNA&Env and DNA vaccinated animals. Our results suggest that combined RepAd priming with ALVAC/Env or DNA&Env regimen boosting might induce potent, functional, long-lasting systemic and mucosal SIV-specific antibodies.

Full Text

Duke Authors

Cited Authors

  • Vargas-Inchaustegui, DA; Tuero, I; Mohanram, V; Musich, T; Pegu, P; Valentin, A; Sui, Y; Rosati, M; Bear, J; Venzon, DJ; Kulkarni, V; Alicea, C; Pilkington, GR; Liyanage, NPM; Demberg, T; Gordon, SN; Wang, Y; Hogg, AE; Frey, B; Patterson, LJ; DiPasquale, J; Montefiori, DC; Sardesai, NY; Reed, SG; Berzofsky, JA; Franchini, G; Felber, BK; Pavlakis, GN; Robert-Guroff, M

Published Date

  • August 2014

Published In

Volume / Issue

  • 153 / 2

Start / End Page

  • 308 - 322

PubMed ID

  • 24907411

Pubmed Central ID

  • 24907411

Electronic International Standard Serial Number (EISSN)

  • 1521-7035

Digital Object Identifier (DOI)

  • 10.1016/j.clim.2014.05.008

Language

  • eng

Conference Location

  • United States