Measurement of fatty acid oxidation rates in animal tissues and cell lines.


Journal Article

While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.

Full Text

Duke Authors

Cited Authors

  • Huynh, FK; Green, MF; Koves, TR; Hirschey, MD

Published Date

  • 2014

Published In

Volume / Issue

  • 542 /

Start / End Page

  • 391 - 405

PubMed ID

  • 24862277

Pubmed Central ID

  • 24862277

Electronic International Standard Serial Number (EISSN)

  • 1557-7988

Digital Object Identifier (DOI)

  • 10.1016/B978-0-12-416618-9.00020-0


  • eng

Conference Location

  • United States