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Measurement of fatty acid oxidation rates in animal tissues and cell lines.

Publication ,  Journal Article
Huynh, FK; Green, MF; Koves, TR; Hirschey, MD
Published in: Methods Enzymol
2014

While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.

Duke Scholars

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2014

Volume

542

Start / End Page

391 / 405

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Palmitates
  • Oxidation-Reduction
  • Fatty Acids
  • Cell Line
  • Carbon Radioisotopes
  • Biochemistry & Molecular Biology
  • Biochemistry
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huynh, F. K., Green, M. F., Koves, T. R., & Hirschey, M. D. (2014). Measurement of fatty acid oxidation rates in animal tissues and cell lines. Methods Enzymol, 542, 391–405. https://doi.org/10.1016/B978-0-12-416618-9.00020-0
Huynh, Frank K., Michelle F. Green, Timothy R. Koves, and Matthew D. Hirschey. “Measurement of fatty acid oxidation rates in animal tissues and cell lines.Methods Enzymol 542 (2014): 391–405. https://doi.org/10.1016/B978-0-12-416618-9.00020-0.
Huynh FK, Green MF, Koves TR, Hirschey MD. Measurement of fatty acid oxidation rates in animal tissues and cell lines. Methods Enzymol. 2014;542:391–405.
Huynh, Frank K., et al. “Measurement of fatty acid oxidation rates in animal tissues and cell lines.Methods Enzymol, vol. 542, 2014, pp. 391–405. Pubmed, doi:10.1016/B978-0-12-416618-9.00020-0.
Huynh FK, Green MF, Koves TR, Hirschey MD. Measurement of fatty acid oxidation rates in animal tissues and cell lines. Methods Enzymol. 2014;542:391–405.

Published In

Methods Enzymol

DOI

EISSN

1557-7988

Publication Date

2014

Volume

542

Start / End Page

391 / 405

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Palmitates
  • Oxidation-Reduction
  • Fatty Acids
  • Cell Line
  • Carbon Radioisotopes
  • Biochemistry & Molecular Biology
  • Biochemistry
  • Animals
  • 3101 Biochemistry and cell biology