Genetic Evidence of Importation of Drug-Resistant Plasmodium falciparum to Guatemala from the Democratic Republic of the Congo.

Journal Article (Journal Article)

Imported malaria threatens control and elimination efforts in countries that have low rates of transmission. In 2010, an outbreak of Plasmodium falciparum malaria was reported among United Nations peacekeeping soldiers from Guatemala who had recently returned from the Democratic Republic of the Congo (DRC). Epidemiologic evidence suggested that the soldiers were infected in the DRC, but local transmission could not be ruled out in all cases. We used population genetic analyses of neutral microsatellites to determine the outbreak source. Genetic relatedness was compared among parasites found in samples from the soldiers and parasite populations collected in the DRC and Guatemala; parasites identified in the soldiers were more closely related to those from the DRC. A phylogenetic clustering analysis confirms this identification with >99.9% confidence. Thus, results support the hypothesis that the soldiers likely imported malaria from the DRC. This study demonstrates the utility of molecular genotyping in outbreak investigations.

Full Text

Duke Authors

Cited Authors

  • Patel, JC; Taylor, SM; Juliao, PC; Parobek, CM; Janko, M; Gonzalez, LD; Ortiz, L; Padilla, N; Tshefu, AK; Emch, M; Udhayakumar, V; Lindblade, K; Meshnick, SR

Published Date

  • June 2014

Published In

Volume / Issue

  • 20 / 6

Start / End Page

  • 932 - 940

PubMed ID

  • 24856348

Pubmed Central ID

  • PMC4036788

Electronic International Standard Serial Number (EISSN)

  • 1080-6059

Digital Object Identifier (DOI)

  • 10.3201/eid2006.131204


  • eng

Conference Location

  • United States