Inter-laboratory validation of bioaccessibility testing for metals.


Journal Article

Bioelution assays are fast, simple alternatives to in vivo testing. In this study, the intra- and inter-laboratory variability in bioaccessibility data generated by bioelution tests were evaluated in synthetic fluids relevant to oral, inhalation, and dermal exposure. Using one defined protocol, five laboratories measured metal release from cobalt oxide, cobalt powder, copper concentrate, Inconel alloy, leaded brass alloy, and nickel sulfate hexahydrate. Standard deviations of repeatability (sr) and reproducibility (sR) were used to evaluate the intra- and inter-laboratory variability, respectively. Examination of the sR:sr ratios demonstrated that, while gastric and lysosomal fluids had reasonably good reproducibility, other fluids did not show as good concordance between laboratories. Relative standard deviation (RSD) analysis showed more favorable reproducibility outcomes for some data sets; overall results varied more between- than within-laboratories. RSD analysis of sr showed good within-laboratory variability for all conditions except some metals in interstitial fluid. In general, these findings indicate that absolute bioaccessibility results in some biological fluids may vary between different laboratories. However, for most applications, measures of relative bioaccessibility are needed, diminishing the requirement for high inter-laboratory reproducibility in absolute metal releases. The inter-laboratory exercise suggests that the degrees of freedom within the protocol need to be addressed.

Full Text

Cited Authors

  • Henderson, RG; Verougstraete, V; Anderson, K; Arbildua, JJ; Brock, TO; Brouwers, T; Cappellini, D; Delbeke, K; Herting, G; Hixon, G; Odnevall Wallinder, I; Rodriguez, PH; Van Assche, F; Wilrich, P; Oller, AR

Published Date

  • October 2014

Published In

Volume / Issue

  • 70 / 1

Start / End Page

  • 170 - 181

PubMed ID

  • 24979734

Pubmed Central ID

  • 24979734

Electronic International Standard Serial Number (EISSN)

  • 1096-0295

International Standard Serial Number (ISSN)

  • 0273-2300

Digital Object Identifier (DOI)

  • 10.1016/j.yrtph.2014.06.021


  • eng