Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients.
Journal Article (Journal Article)
A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.
Full Text
Duke Authors
Cited Authors
- Mikita, K; Thakur, K; Anstey, NM; Piera, KA; Pardo, CA; Weinberg, JB; Mukemba, J; Florence, S; Mwaikambo, ED; Granger, DL; Sullivan, DJ
Published Date
- September 2014
Published In
Volume / Issue
- 91 / 3
Start / End Page
- 486 - 492
PubMed ID
- 24980497
Pubmed Central ID
- PMC4155548
Electronic International Standard Serial Number (EISSN)
- 1476-1645
Digital Object Identifier (DOI)
- 10.4269/ajtmh.14-0210
Language
- eng
Conference Location
- United States