Invisible RNA state dynamically couples distant motifs.


Journal Article

Using on- and off-resonance carbon and nitrogen R1ρ NMR relaxation dispersion in concert with mutagenesis and NMR chemical shift fingerprinting, we show that the transactivation response element RNA from the HIV-1 exists in dynamic equilibrium with a transient state that has a lifetime of ∼2 ms and population of ∼0.4%, which simultaneously remodels the structure of a bulge, stem, and apical loop. This is accomplished by a global change in strand register, in which bulge residues pair up with residues in the upper stem, causing a reshuffling of base pairs that propagates to the tip of apical loop, resulting in the creation of three noncanonical base pairs. Our results show that transient states can remodel distant RNA motifs and possibly give rise to mechanisms for rapid long-range communication in RNA that can be harnessed in processes such as cooperative folding and ribonucleoprotein assembly.

Full Text

Duke Authors

Cited Authors

  • Lee, J; Dethoff, EA; Al-Hashimi, HM

Published Date

  • July 1, 2014

Published In

Volume / Issue

  • 111 / 26

Start / End Page

  • 9485 - 9490

PubMed ID

  • 24979799

Pubmed Central ID

  • 24979799

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1407969111


  • eng

Conference Location

  • United States