Hypothesis-independent pathway analysis implicates GABA and acetyl-CoA metabolism in primary open-angle glaucoma and normal-pressure glaucoma.

Journal Article (Journal Article)

Primary open-angle glaucoma (POAG) is a leading cause of blindness worldwide. Using genome-wide association single-nucleotide polymorphism data from the Glaucoma Genes and Environment study and National Eye Institute Glaucoma Human Genetics Collaboration comprising 3,108 cases and 3,430 controls, we assessed biologic pathways as annotated in the KEGG database for association with risk of POAG. After correction for genic overlap among pathways, we found 4 pathways, butanoate metabolism (hsa00650), hematopoietic cell lineage (hsa04640), lysine degradation (hsa00310) and basal transcription factors (hsa03022) related to POAG with permuted p < 0.001. In addition, the human leukocyte antigen (HLA) gene family was significantly associated with POAG (p < 0.001). In the POAG subset with normal-pressure glaucoma (NPG), the butanoate metabolism pathway was also significantly associated (p < 0.001) as well as the MAPK and Hedgehog signaling pathways (hsa04010 and hsa04340), glycosaminoglycan biosynthesis-heparan sulfate pathway (hsa00534) and the phenylalanine, tyrosine and tryptophan biosynthesis pathway (hsa0400). The butanoate metabolism pathway overall, and specifically the aspects of the pathway that contribute to GABA and acetyl-CoA metabolism, was the only pathway significantly associated with both POAG and NPG. Collectively these results implicate GABA and acetyl-CoA metabolism in glaucoma pathogenesis, and suggest new potential therapeutic targets.

Full Text

Duke Authors

Cited Authors

  • Bailey, JNC; Yaspan, BL; Pasquale, LR; Hauser, MA; Kang, JH; Loomis, SJ; Brilliant, M; Budenz, DL; Christen, WG; Fingert, J; Gaasterland, D; Gaasterland, T; Kraft, P; Lee, RK; Lichter, PR; Liu, Y; McCarty, CA; Moroi, SE; Richards, JE; Realini, T; Schuman, JS; Scott, WK; Singh, K; Sit, AJ; Vollrath, D; Wollstein, G; Zack, DJ; Zhang, K; Pericak-Vance, MA; Allingham, RR; Weinreb, RN; Haines, JL; Wiggs, JL

Published Date

  • October 2014

Published In

Volume / Issue

  • 133 / 10

Start / End Page

  • 1319 - 1330

PubMed ID

  • 25037249

Pubmed Central ID

  • PMC4273559

Electronic International Standard Serial Number (EISSN)

  • 1432-1203

Digital Object Identifier (DOI)

  • 10.1007/s00439-014-1468-7


  • eng

Conference Location

  • Germany