A study of axonal protein trafficking in neuronal networks via the microfluidic platform

Journal Article

This paper reports a microfluidic platform for culturing neuronal networks and the study of axonal protein trafficking. The device consists of cultural microchambers connected by microchannel array. Molecular biological methods were used to express fluorescent proteins in neurons. Preliminary results show that the neurons can be polarized with their soma and axons being compartmentalized into different fluidically isolated microenvironments. When chemical stimulation was applied to axonal chamber, anterograde migration of expressed fluorescent proteins could be observed. The platform will be useful in neuroscience research for studying the response of individual neurons to extracellular stimulation.

Duke Authors

Cited Authors

  • Fu, Y; Vandongen, A; Bourouina, T; Tsang, WM; Je, M; Liu, AQ

Published Date

  • January 1, 2012

Published In

  • Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Microtas 2012

Start / End Page

  • 1072 - 1074

Citation Source

  • Scopus