Visualization of arrestin recruitment by a G-protein-coupled receptor.

Published

Journal Article

G-protein-coupled receptors (GPCRs) are critically regulated by β-arrestins, which not only desensitize G-protein signalling but also initiate a G-protein-independent wave of signalling. A recent surge of structural data on a number of GPCRs, including the β2 adrenergic receptor (β2AR)-G-protein complex, has provided novel insights into the structural basis of receptor activation. However, complementary information has been lacking on the recruitment of β-arrestins to activated GPCRs, primarily owing to challenges in obtaining stable receptor-β-arrestin complexes for structural studies. Here we devised a strategy for forming and purifying a functional human β2AR-β-arrestin-1 complex that allowed us to visualize its architecture by single-particle negative-stain electron microscopy and to characterize the interactions between β2AR and β-arrestin 1 using hydrogen-deuterium exchange mass spectrometry (HDX-MS) and chemical crosslinking. Electron microscopy two-dimensional averages and three-dimensional reconstructions reveal bimodal binding of β-arrestin 1 to the β2AR, involving two separate sets of interactions, one with the phosphorylated carboxy terminus of the receptor and the other with its seven-transmembrane core. Areas of reduced HDX together with identification of crosslinked residues suggest engagement of the finger loop of β-arrestin 1 with the seven-transmembrane core of the receptor. In contrast, focal areas of raised HDX levels indicate regions of increased dynamics in both the N and C domains of β-arrestin 1 when coupled to the β2AR. A molecular model of the β2AR-β-arrestin signalling complex was made by docking activated β-arrestin 1 and β2AR crystal structures into the electron microscopy map densities with constraints provided by HDX-MS and crosslinking, allowing us to obtain valuable insights into the overall architecture of a receptor-arrestin complex. The dynamic and structural information presented here provides a framework for better understanding the basis of GPCR regulation by arrestins.

Full Text

Duke Authors

Cited Authors

  • Shukla, AK; Westfield, GH; Xiao, K; Reis, RI; Huang, L-Y; Tripathi-Shukla, P; Qian, J; Li, S; Blanc, A; Oleskie, AN; Dosey, AM; Su, M; Liang, C-R; Gu, L-L; Shan, J-M; Chen, X; Hanna, R; Choi, M; Yao, XJ; Klink, BU; Kahsai, AW; Sidhu, SS; Koide, S; Penczek, PA; Kossiakoff, AA; Woods, VL; Kobilka, BK; Skiniotis, G; Lefkowitz, RJ

Published Date

  • August 14, 2014

Published In

Volume / Issue

  • 512 / 7513

Start / End Page

  • 218 - 222

PubMed ID

  • 25043026

Pubmed Central ID

  • 25043026

Electronic International Standard Serial Number (EISSN)

  • 1476-4687

Digital Object Identifier (DOI)

  • 10.1038/nature13430

Language

  • eng

Conference Location

  • England