A radioisotope based methodology for plant-fungal interactions in the rhizosphere


Journal Article

In plant ecophysiology research there is interest in studying the biology of the rhizosphere because of its importance in plant nutrient-interactions. The rhizosphere is the zone of soil surrounding a plant's root system where microbes (such as fungi) are influenced by the root and the roots by the microbes. We are investigating a methodology for imaging the distribution of molecular compounds of interest in the rhizosphere without disturbing the root or soil habitat. Our intention is to develop a single photon emission computed tomography (SPECT) system (PhytoSPECT) to image the bio-distribution of fungi in association with a host plant's roots. The technique we are exploring makes use of radioactive isotopes as tracers to label molecules that bind to fungal-specific compounds of interest and to image the fungi distribution in the plant and/or soil. We report on initial experiments designed to test the ability of fungal-specific compounds labeled with an iodine radioisotope that binds to chitin monomers (N-acetylglucosamine). Chitin is a compound not found in roots but in fungal cell walls. We will test the ability to label the compound with radioactive isotopes of iodine (125I, and123I). © 2013 IEEE.

Full Text

Duke Authors

Cited Authors

  • Weisenberger, AG; Bonito, G; Lee, S; McKisson, JE; Gryganskyi, A; Reid, CD; Smith, MF; Vaidyanathan, G; Welch, B

Published Date

  • January 1, 2013

Published In

International Standard Serial Number (ISSN)

  • 1095-7863

Digital Object Identifier (DOI)

  • 10.1109/NSSMIC.2013.6829457

Citation Source

  • Scopus