Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs.
Micropatterning is a powerful technique to control cell shape and position on a culture substrate. In this chapter, we describe the method to reproducibly create large numbers of micropatterned heterotypic cell pairs with defined size, shape, and length of cell-cell contact. These cell pairs can be utilized in patch clamp recordings to quantify electrical interactions between cardiomyocytes and non-cardiomyocytes.
Nguyen, H; Badie, N; McSpadden, L; Pedrotty, D; Bursac, N
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