Protein-DNA binding: complexities and multi-protein codes.

Published

Journal Article (Review)

Binding of proteins to particular DNA sites across the genome is a primary determinant of specificity in genome maintenance and gene regulation. DNA-binding specificity is encoded at multiple levels, from the detailed biophysical interactions between proteins and DNA, to the assembly of multi-protein complexes. At each level, variation in the mechanisms used to achieve specificity has led to difficulties in constructing and applying simple models of DNA binding. We review the complexities in protein-DNA binding found at multiple levels and discuss how they confound the idea of simple recognition codes. We discuss the impact of new high-throughput technologies for the characterization of protein-DNA binding, and how these technologies are uncovering new complexities in protein-DNA recognition. Finally, we review the concept of multi-protein recognition codes in which new DNA-binding specificities are achieved by the assembly of multi-protein complexes.

Full Text

Duke Authors

Cited Authors

  • Siggers, T; Gordân, R

Published Date

  • February 2014

Published In

Volume / Issue

  • 42 / 4

Start / End Page

  • 2099 - 2111

PubMed ID

  • 24243859

Pubmed Central ID

  • 24243859

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Digital Object Identifier (DOI)

  • 10.1093/nar/gkt1112

Language

  • eng

Conference Location

  • England