Protein-DNA binding: complexities and multi-protein codes.
Binding of proteins to particular DNA sites across the genome is a primary determinant of specificity in genome maintenance and gene regulation. DNA-binding specificity is encoded at multiple levels, from the detailed biophysical interactions between proteins and DNA, to the assembly of multi-protein complexes. At each level, variation in the mechanisms used to achieve specificity has led to difficulties in constructing and applying simple models of DNA binding. We review the complexities in protein-DNA binding found at multiple levels and discuss how they confound the idea of simple recognition codes. We discuss the impact of new high-throughput technologies for the characterization of protein-DNA binding, and how these technologies are uncovering new complexities in protein-DNA recognition. Finally, we review the concept of multi-protein recognition codes in which new DNA-binding specificities are achieved by the assembly of multi-protein complexes.
Duke Scholars
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- Protein Binding
- Developmental Biology
- DNA-Binding Proteins
- DNA
- 41 Environmental sciences
- 34 Chemical sciences
- 31 Biological sciences
- 08 Information and Computing Sciences
- 06 Biological Sciences
- 05 Environmental Sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Protein Binding
- Developmental Biology
- DNA-Binding Proteins
- DNA
- 41 Environmental sciences
- 34 Chemical sciences
- 31 Biological sciences
- 08 Information and Computing Sciences
- 06 Biological Sciences
- 05 Environmental Sciences