Cooperation of B cell lineages in induction of HIV-1-broadly neutralizing antibodies

Journal Article

Development of strategies for induction of HIV-1 broadly neutralizing antibodies (bnAbs) by vaccines is a priority. Determining the steps of bnAb induction in HIV-1-infected individuals who make bnAbs is a key strategy for immunogen design. Here, we study the B cell response in a bnAb-producing individual and report cooperation between two B cell lineages to drive bnAb development. We isolated a virus-neutralizing antibody lineage that targeted an envelope region (loop D) and selected virus escape mutants that resulted in both enhanced bnAb lineage envelope binding and escape mutant neutralization - traits associated with increased B cell antigen drive. Thus, in this individual, two B cell lineages cooperated to induce the development of bnAbs. Design of vaccine immunogens that simultaneously drive both helper and broadly neutralizing B cell lineages may be important for vaccine-induced recapitulation of events that transpire during the maturation of neutralizing antibodies in HIV-1-infected individuals. © 2014 Elsevier Inc.

Full Text

Duke Authors

Cited Authors

  • Gao, F; Bonsignori, M; Liao, HX; Kumar, A; Xia, SM; Lu, X; Cai, F; Hwang, KK; Song, H; Zhou, T; Lynch, RM; Alam, SM; Moody, MA; Ferrari, G; Berrong, M; Kelsoe, G; Shaw, GM; Hahn, BH; Montefiori, DC; Kamanga, G; Cohen, MS; Hraber, P; Kwong, PD; Korber, BT; Mascola, JR; Kepler, TB; Haynes, BF

Published Date

  • July 31, 2014

Published In

Volume / Issue

  • 158 / 3

Start / End Page

  • 481 - 491

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2014.06.022

Citation Source

  • Scopus