Relationship between simian virus 40 large tumor antigen expression and tumor formation in transgenic mice.

Published

Journal Article

A line of transgenic mice containing the simian virus 40 (SV40) large tumor antigen gene under the control of the viral enhancer-promoter expressed this viral protein in the brains of these mice within the first 2 weeks after birth. Multiple foci of anaplastic cells formed in the choroid plexuses of these mice at 36 to 41 days after birth, and normal tissue coexisted with these transformed foci. Immunoperoxidase staining to detect the SV40 T antigen showed tumor-specific expression of nuclear T antigen at late times in tumor development, approximately 90 to 100 days and thereafter. The level of SV40 T antigen, on a per cell basis, appeared to be lower in the great majority of choroid plexus cells at earlier times in tumor development. These results suggest that low levels of tumor antigen (14 to 36 days) are present before detectable pathology (36 to 41 days) and the level of T antigen per cell is higher in rapidly growing late-stage tumors (older than 90 days).

Full Text

Duke Authors

Cited Authors

  • Van Dyke, TA; Finlay, C; Miller, D; Marks, J; Lozano, G; Levine, AJ

Published Date

  • June 1, 1987

Published In

Volume / Issue

  • 61 / 6

Start / End Page

  • 2029 - 2032

PubMed ID

  • 3033329

Pubmed Central ID

  • 3033329

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States