Phenotypic signatures arising from unbalanced bacterial growth.

Published

Journal Article

Fluctuations in the growth rate of a bacterial culture during unbalanced growth are generally considered undesirable in quantitative studies of bacterial physiology. Under well-controlled experimental conditions, however, these fluctuations are not random but instead reflect the interplay between intra-cellular networks underlying bacterial growth and the growth environment. Therefore, these fluctuations could be considered quantitative phenotypes of the bacteria under a specific growth condition. Here, we present a method to identify "phenotypic signatures" by time-frequency analysis of unbalanced growth curves measured with high temporal resolution. The signatures are then applied to differentiate amongst different bacterial strains or the same strain under different growth conditions, and to identify the essential architecture of the gene network underlying the observed growth dynamics. Our method has implications for both basic understanding of bacterial physiology and for the classification of bacterial strains.

Full Text

Duke Authors

Cited Authors

  • Tan, C; Smith, RP; Tsai, M-C; Schwartz, R; You, L

Published Date

  • August 7, 2014

Published In

Volume / Issue

  • 10 / 8

Start / End Page

  • e1003751 -

PubMed ID

  • 25101949

Pubmed Central ID

  • 25101949

Electronic International Standard Serial Number (EISSN)

  • 1553-7358

International Standard Serial Number (ISSN)

  • 1553-734X

Digital Object Identifier (DOI)

  • 10.1371/journal.pcbi.1003751

Language

  • eng