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Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development.

Publication ,  Journal Article
Tschowri, N; Schumacher, MA; Schlimpert, S; Chinnam, NB; Findlay, KC; Brennan, RG; Buttner, MJ
Published in: Cell
August 28, 2014

The cyclic dinucleotide c-di-GMP is a signaling molecule with diverse functions in cellular physiology. Here, we report that c-di-GMP can assemble into a tetramer that mediates the effective dimerization of a transcription factor, BldD, which controls the progression of multicellular differentiation in sporulating actinomycete bacteria. BldD represses expression of sporulation genes during vegetative growth in a manner that depends on c-di-GMP-mediated dimerization. Structural and biochemical analyses show that tetrameric c-di-GMP links two subunits of BldD through their C-terminal domains, which are otherwise separated by ~10 Å and thus cannot effect dimerization directly. Binding of the c-di-GMP tetramer by BldD is selective and requires a bipartite RXD-X8-RXXD signature. The findings indicate a unique mechanism of protein dimerization and the ability of nucleotide signaling molecules to assume alternative oligomeric states to effect different functions.

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Published In

Cell

DOI

EISSN

1097-4172

Publication Date

August 28, 2014

Volume

158

Issue

5

Start / End Page

1136 / 1147

Location

United States

Related Subject Headings

  • Transcription Factors
  • Streptomyces
  • Spores, Bacterial
  • Sequence Alignment
  • Molecular Sequence Data
  • Models, Molecular
  • Dimerization
  • Developmental Biology
  • Cyclic GMP
  • Crystallography, X-Ray
 

Citation

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Tschowri, N., Schumacher, M. A., Schlimpert, S., Chinnam, N. B., Findlay, K. C., Brennan, R. G., & Buttner, M. J. (2014). Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development. Cell, 158(5), 1136–1147. https://doi.org/10.1016/j.cell.2014.07.022
Tschowri, Natalia, Maria A. Schumacher, Susan Schlimpert, Naga Babu Chinnam, Kim C. Findlay, Richard G. Brennan, and Mark J. Buttner. “Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development.Cell 158, no. 5 (August 28, 2014): 1136–47. https://doi.org/10.1016/j.cell.2014.07.022.
Tschowri N, Schumacher MA, Schlimpert S, Chinnam NB, Findlay KC, Brennan RG, et al. Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development. Cell. 2014 Aug 28;158(5):1136–47.
Tschowri, Natalia, et al. “Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development.Cell, vol. 158, no. 5, Aug. 2014, pp. 1136–47. Pubmed, doi:10.1016/j.cell.2014.07.022.
Tschowri N, Schumacher MA, Schlimpert S, Chinnam NB, Findlay KC, Brennan RG, Buttner MJ. Tetrameric c-di-GMP mediates effective transcription factor dimerization to control Streptomyces development. Cell. 2014 Aug 28;158(5):1136–1147.
Journal cover image

Published In

Cell

DOI

EISSN

1097-4172

Publication Date

August 28, 2014

Volume

158

Issue

5

Start / End Page

1136 / 1147

Location

United States

Related Subject Headings

  • Transcription Factors
  • Streptomyces
  • Spores, Bacterial
  • Sequence Alignment
  • Molecular Sequence Data
  • Models, Molecular
  • Dimerization
  • Developmental Biology
  • Cyclic GMP
  • Crystallography, X-Ray