Skip to main content

A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation.

Publication ,  Journal Article
McGee, MJ; Danziger, ZC; Bamford, JA; Grill, WM
Published in: American journal of physiology. Renal physiology
October 2014

Electrical stimulation of pudendal afferents can inhibit bladder contractions and increase bladder capacity. Recent results suggest that stimulation-evoked bladder inhibition is mediated by a mechanism other than activation of sympathetic bladder efferents in the hypogastric nerve, generating α-adrenergic receptor-mediated inhibition at the vesical ganglia and/or β-adrenergic receptor-mediated direct inhibition of the detrusor muscle. We investigated several inhibitory neurotransmitters that may instead be necessary for stimulation-evoked inhibition and found that intravenous picrotoxin, a noncompetitive GABAA antagonist, significantly and reversibly blocked pudendal afferent stimulation-evoked inhibition of bladder contractions in a dose-dependent manner. Similarly, intravenous picrotoxin also blocked pudendal afferent stimulation-evoked inhibition of nociceptive bladder contractions evoked by acetic acid infusion. Furthermore, intrathecal administration of picrotoxin at the lumbosacral spinal cord also blocked bladder inhibition by pudendal afferent stimulation. On the other hand, glycinergic, adrenergic, or opioidergic mechanisms were not necessary for bladder inhibition evoked by pudendal afferent stimulation. These results identify a lumbosacral spinal GABAergic mechanism of bladder inhibition evoked by pudendal afferent stimulation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

American journal of physiology. Renal physiology

DOI

EISSN

1522-1466

ISSN

1931-857X

Publication Date

October 2014

Volume

307

Issue

8

Start / End Page

F921 / F930

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Receptors, Adrenergic, beta
  • Pudendal Nerve
  • Picrotoxin
  • Penis
  • Neurons, Afferent
  • Muscle Contraction
  • Male
  • GABA-A Receptor Antagonists
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McGee, M. J., Danziger, Z. C., Bamford, J. A., & Grill, W. M. (2014). A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation. American Journal of Physiology. Renal Physiology, 307(8), F921–F930. https://doi.org/10.1152/ajprenal.00330.2014
McGee, Meredith J., Zachary C. Danziger, Jeremy A. Bamford, and Warren M. Grill. “A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation.American Journal of Physiology. Renal Physiology 307, no. 8 (October 2014): F921–30. https://doi.org/10.1152/ajprenal.00330.2014.
McGee MJ, Danziger ZC, Bamford JA, Grill WM. A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation. American journal of physiology Renal physiology. 2014 Oct;307(8):F921–30.
McGee, Meredith J., et al. “A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation.American Journal of Physiology. Renal Physiology, vol. 307, no. 8, Oct. 2014, pp. F921–30. Epmc, doi:10.1152/ajprenal.00330.2014.
McGee MJ, Danziger ZC, Bamford JA, Grill WM. A spinal GABAergic mechanism is necessary for bladder inhibition by pudendal afferent stimulation. American journal of physiology Renal physiology. 2014 Oct;307(8):F921–F930.

Published In

American journal of physiology. Renal physiology

DOI

EISSN

1522-1466

ISSN

1931-857X

Publication Date

October 2014

Volume

307

Issue

8

Start / End Page

F921 / F930

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Receptors, Adrenergic, beta
  • Pudendal Nerve
  • Picrotoxin
  • Penis
  • Neurons, Afferent
  • Muscle Contraction
  • Male
  • GABA-A Receptor Antagonists