The significance of nuclear deformation for cancer cell transmigration
In this paper, we present a microfluidic platform for the study of cancer cell transmigration and anti-cancer drug screening. The device consists of an array of microchannels with dimensions close to the capillaries on the cellular barriers, while a thin glass coverslip is used as the substrate to enable high resolution imaging of cell dynamics during cancer cell transmigration. We have investigated the transmigration of highly metastatic human breast cancer cells, MDA-MB-231, after their cell nucleus are fluorescently labeled. The results show that 1) the cell nucleus deformed drastically during the transmigration process, and 2) an anti-cancer drug targeting the cell nucleus significantly inhibited transmigration.
Fu, Y; Vandongen, A; Bourouina, T; Tsang, WM; Je, M; Liu, AQ
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Microtas 2012
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