Salinity-dependent silver nanoparticle uptake and transformation by Atlantic killifish (Fundulus heteroclitus) embryos.

Journal Article (Journal Article)

We assessed the biodistribution and in situ speciation of sub-lethal concentrations of citrate-coated silver nanoparticles and dissolved silver within Fundulus heteroclitus embryos. Using a thorough physico-chemical characterization, we studied the role of salinity on both uptake and in situ speciation. The Ag uptake or adsorption on the chorion was reduced by 2.3-fold for Ag NPs, and 2.9-fold for AgNO3 in estuarine water (10‰ ASW) compared to deionized water (0‰ ASW). Between 58% and 85% of the silver was localized on/in the chorion and formed patches between 20 and 80 µm. More than a physical barrier, the chorion was found to be a chemically reactive membrane controlling the in situ speciation of silver. A strong complexation of the Cit-Ag NPs with the thiolated groups of proteins or enzymes of the chorion was responsible for the oxidation of 48 ± 5% of the Ag(0) into Ag((I))-S species at 0‰ ASW. However, at 10‰ ASW, the presence of Cl(-) ions at the surface of Ag NPs slow down this oxidation. For the dissolved silver, we observed that in deionized water 69 ± 7% of Ag(+) taken up by the chorion was complexed by the thiolated molecules while the others 30 ± 3% were reduced into Ag(0) likely via interaction with the hemiacetal-reducing ends of polysaccharides of the chorion.

Full Text

Duke Authors

Cited Authors

  • Auffan, M; Matson, CW; Rose, J; Arnold, M; Proux, O; Fayard, B; Liu, W; Chaurand, P; Wiesner, MR; Bottero, J-Y; Di Giulio, RT

Published Date

  • August 2014

Published In

Volume / Issue

  • 8 Suppl 1 /

Start / End Page

  • 167 - 176

PubMed ID

  • 24359331

Electronic International Standard Serial Number (EISSN)

  • 1743-5404

International Standard Serial Number (ISSN)

  • 1743-5390

Digital Object Identifier (DOI)

  • 10.3109/17435390.2013.869627


  • eng