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MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans.

Publication ,  Journal Article
Wang, L; Shen, W; Lei, S; Matus, D; Sherwood, D; Wang, Z
Published in: Biochemical and biophysical research communications
September 2014

Cell invasion through basement membrane (BM) occurs in many physiological and pathological contexts. MIG-10, the Caenorhabditis elegans Lamellipodin (Lpd), regulates diverse biological processes. Its function and regulation in cell invasive behavior remain unclear. Using anchor cell (AC) invasion in C. elegans as an in vivo invasion model, we have previously found that mig-10's activity is largely outside of UNC-6 (netrin) signaling, a chemical cue directing AC invasion. We have shown that MIG-10 is a target of the transcription factor FOS-1A and facilitates BM breaching. Combining genetics and imaging analyses, we report that MIG-10 synergizes with UNC-6 to promote AC attachment to the BM, revealing a functional role for MIG-10 in stabilizing AC-BM adhesion. MIG-10 is also required for F-actin accumulation in the absence of UNC-6. Further, we identify mig-10 as a transcriptional target negatively regulated by EGL-43A (C. elegans Evi-1 proto-oncogene), a transcription factor positively controlled by FOS-1A. The revelation of this negative regulation unmasks an incoherent feedforward circuit existing among fos-1, egl-43 and mig-10. Moreover, our study suggests the functional importance of the negative regulation on mig-10 expression by showing that excessive MIG-10 impairs AC invasion. Thus, we provide new insight into MIG-10's function and its complex transcriptional regulation during cell invasive behavior.

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Published In

Biochemical and biophysical research communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

September 2014

Volume

452

Issue

3

Start / End Page

328 / 333

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Netrins
  • Nerve Tissue Proteins
  • Gonads
  • Gene Expression Regulation
  • Disorders of Sex Development
  • Cell Movement
 

Citation

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Wang, L., Shen, W., Lei, S., Matus, D., Sherwood, D., & Wang, Z. (2014). MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans. Biochemical and Biophysical Research Communications, 452(3), 328–333. https://doi.org/10.1016/j.bbrc.2014.08.049
Wang, Lin, Wanqing Shen, Shijun Lei, David Matus, David Sherwood, and Zheng Wang. “MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans.Biochemical and Biophysical Research Communications 452, no. 3 (September 2014): 328–33. https://doi.org/10.1016/j.bbrc.2014.08.049.
Wang L, Shen W, Lei S, Matus D, Sherwood D, Wang Z. MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans. Biochemical and biophysical research communications. 2014 Sep;452(3):328–33.
Wang, Lin, et al. “MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans.Biochemical and Biophysical Research Communications, vol. 452, no. 3, Sept. 2014, pp. 328–33. Epmc, doi:10.1016/j.bbrc.2014.08.049.
Wang L, Shen W, Lei S, Matus D, Sherwood D, Wang Z. MIG-10 (Lamellipodin) stabilizes invading cell adhesion to basement membrane and is a negative transcriptional target of EGL-43 in C. elegans. Biochemical and biophysical research communications. 2014 Sep;452(3):328–333.
Journal cover image

Published In

Biochemical and biophysical research communications

DOI

EISSN

1090-2104

ISSN

0006-291X

Publication Date

September 2014

Volume

452

Issue

3

Start / End Page

328 / 333

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Signal Transduction
  • Proto-Oncogene Proteins c-fos
  • Netrins
  • Nerve Tissue Proteins
  • Gonads
  • Gene Expression Regulation
  • Disorders of Sex Development
  • Cell Movement