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Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins.

Publication ,  Journal Article
Del Prete, GQ; Eilers, B; Moldt, B; Keele, BF; Estes, JD; Rodriguez, A; Sampias, M; Oswald, K; Fast, R; Trubey, CM; Chertova, E; Smedley, J ...
Published in: Cell Host Microbe
September 10, 2014

Infection of macaques with chimeric viruses based on SIVMAC but expressing the HIV-1 envelope (Env) glycoproteins (SHIVs) remains the most powerful model for evaluating prevention and therapeutic strategies against AIDS. Unfortunately, only a few SHIVs are currently available. Furthermore, their generation has required extensive adaptation of the HIV-1 Env sequences in macaques so they may not accurately represent HIV-1 Env proteins circulating in humans, potentially limiting their translational utility. We developed a strategy for generating large numbers of SHIV constructs expressing Env proteins from newly transmitted HIV-1 strains. By inoculating macaques with cocktails of multiple SHIV variants, we selected SHIVs that can replicate and cause AIDS-like disease in immunologically intact rhesus macaques without requiring animal-to-animal passage. One of these SHIVs could be transmitted mucosally. We demonstrate the utility of the SHIVs generated by this method for evaluating neutralizing antibody administration as a protection against mucosal SHIV challenge.

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Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 10, 2014

Volume

16

Issue

3

Start / End Page

412 / 418

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virus Cultivation
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Macaca
  • Immunology
  • Humans
  • HIV-1
  • HIV Infections
 

Citation

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Del Prete, G. Q., Eilers, B., Moldt, B., Keele, B. F., Estes, J. D., Rodriguez, A., … Hatziioannou, T. (2014). Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host Microbe, 16(3), 412–418. https://doi.org/10.1016/j.chom.2014.08.003
Del Prete, Gregory Q., Braiden Eilers, Brian Moldt, Brandon F. Keele, Jacob D. Estes, Anthony Rodriguez, Marissa Sampias, et al. “Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins.Cell Host Microbe 16, no. 3 (September 10, 2014): 412–18. https://doi.org/10.1016/j.chom.2014.08.003.
Del Prete GQ, Eilers B, Moldt B, Keele BF, Estes JD, Rodriguez A, et al. Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host Microbe. 2014 Sep 10;16(3):412–8.
Del Prete, Gregory Q., et al. “Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins.Cell Host Microbe, vol. 16, no. 3, Sept. 2014, pp. 412–18. Pubmed, doi:10.1016/j.chom.2014.08.003.
Del Prete GQ, Eilers B, Moldt B, Keele BF, Estes JD, Rodriguez A, Sampias M, Oswald K, Fast R, Trubey CM, Chertova E, Smedley J, LaBranche CC, Montefiori DC, Burton DR, Shaw GM, Markowitz M, Piatak M, KewalRamani VN, Bieniasz PD, Lifson JD, Hatziioannou T. Selection of unadapted, pathogenic SHIVs encoding newly transmitted HIV-1 envelope proteins. Cell Host Microbe. 2014 Sep 10;16(3):412–418.
Journal cover image

Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 10, 2014

Volume

16

Issue

3

Start / End Page

412 / 418

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virus Cultivation
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • Macaca
  • Immunology
  • Humans
  • HIV-1
  • HIV Infections