Effects of iron depletion on CALM-AF10 leukemias.

Published

Journal Article

Iron, an essential nutrient for cellular growth and proliferation, enters cells via clathrin-mediated endocytosis. The clathrin assembly lymphoid myeloid (CALM) protein plays an essential role in the cellular import of iron by clathrin-mediated endocytosis. CALM-AF10 leukemias harbor a single copy of the normal CALM gene and therefore may be more sensitive to the growth-inhibitory effect of iron restriction compared with normal hematopoietic cells. We found that CALM heterozygous (CALM(HET)) murine fibroblasts exhibit signs of iron deficiency, with increased surface transferrin receptor levels and reduced growth rates. CALM(HET) hematopoietic cells are more sensitive in vitro to iron chelators than their wild type counterparts. Iron chelation also displayed toxicity toward cultured CALM(HET)CALM-AF10 leukemia cells, and this effect was additive to that of chemotherapy. In mice transplanted with CALM(HET)CALM-AF10 leukemia, we found that dietary iron restriction reduced tumor burden in the spleen. However, dietary iron restriction, used alone or in conjunction with chemotherapy, did not increase survival of mice with CALM(HET)CALM-AF10 leukemia. In summary, although CALM heterozygosity results in iron deficiency and increased sensitivity to iron chelation in vitro, our data in mice do not suggest that iron depletion strategies would be beneficial for the therapy of CALM-AF10 leukemia patients.

Full Text

Duke Authors

Cited Authors

  • Heath, JL; Weiss, JM; Lavau, CP; Wechsler, DS

Published Date

  • December 2014

Published In

Volume / Issue

  • 42 / 12

Start / End Page

  • 1022 - 1030.e1

PubMed ID

  • 25193880

Pubmed Central ID

  • 25193880

Electronic International Standard Serial Number (EISSN)

  • 1873-2399

International Standard Serial Number (ISSN)

  • 0301-472X

Digital Object Identifier (DOI)

  • 10.1016/j.exphem.2014.08.004

Language

  • eng