Development and application of aromatic [(13)C, (1)H] SOFAST-HMQC NMR experiment for nucleic acids.

Journal Article (Journal Article)

Higher sensitivity of NMR spectrometers and novel isotopic labeling schemes have ushered the development of rapid data acquisition methodologies, improving the time resolution with which NMR data can be acquired. For nucleic acids, longitudinal relaxation optimization in conjunction with Ernst angle excitation (SOFAST-HMQC) for imino protons, in addition to rendering rapid pulsing, has been demonstrated to yield significant improvements in sensitivity per unit time. Extending such methodology to other spins offers a viable prospect to measure additional chemical shifts, thereby broadening their utilization for various applications. Here, we introduce the 2D [(13)C, (1)H] aromatic SOFAST-HMQC that results in overall sensitivity gain of 1.4- to 1.7-fold relative to the conventional HMQC and can also be extended to yield long-range heteronuclear chemical shifts such as the adenine imino nitrogens N1, N3, N7 and N9. The applications of these experiments range from monitoring real-time biochemical processes, drug/ligand screening, and to collecting data at very low sample concentration and/or in cases where isotopic enrichment cannot be achieved.

Full Text

Duke Authors

Cited Authors

  • Sathyamoorthy, B; Lee, J; Kimsey, I; Ganser, LR; Al-Hashimi, H

Published Date

  • November 2014

Published In

Volume / Issue

  • 60 / 2-3

Start / End Page

  • 77 - 83

PubMed ID

  • 25186910

Pubmed Central ID

  • PMC4324173

Electronic International Standard Serial Number (EISSN)

  • 1573-5001

Digital Object Identifier (DOI)

  • 10.1007/s10858-014-9856-9


  • eng

Conference Location

  • Netherlands