The statistical conformation of a highly flexible protein: Small-angle x-ray scattering of S. aureus protein A

Journal Article

Summary Staphylococcal protein A (SpA) is a multidomain protein consisting of five globular IgG binding domains separated by a conserved six- to nine-residue flexible linker. We collected SAXS data on the N-terminal protein-binding half of SpA (SpA-N) and constructs consisting of one to five domain modules in order to determine statistical conformation of this important S. aureus virulence factor. We fit the SAXS data to a scattering function based on a new polymer physics model, which provides an analytical description of the SpA-N statistical conformation. We describe a protocol for systematically determining the appropriate level of modeling to fit a SAXS data set based on goodness of fit and whether the addition of parameters improves it. In the case of SpA-N, the analytical polymer physics description provides a depiction of the statistical conformation of a flexible protein that, while lacking atomistic detail, properly reflects the information content of the data. © 2014 Elsevier Ltd.

Full Text

Duke Authors

Cited Authors

  • Capp, JA; Hagarman, A; Richardson, DC; Oas, TG

Published Date

  • 2014

Published In

Volume / Issue

  • 22 / 8

Start / End Page

  • 1184 - 1195

International Standard Serial Number (ISSN)

  • 0969-2126

Digital Object Identifier (DOI)

  • 10.1016/j.str.2014.06.011