Structures of naturally evolved CUP1 tandem arrays in yeast indicate that these arrays are generated by unequal nonhomologous recombination.

Journal Article (Journal Article)

An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural isolates of S. cerevisiae, we identified five different types of CUP1-containing repeats, as well as strains that only had one copy of CUP1. A comparison of the DNA sequences of these strains indicates that the CUP1 tandem arrays were generated by unequal nonhomologous recombination events from strains that had one CUP1 gene.

Full Text

Duke Authors

Cited Authors

  • Zhao, Y; Strope, PK; Kozmin, SG; McCusker, JH; Dietrich, FS; Kokoska, RJ; Petes, TD

Published Date

  • September 17, 2014

Published In

Volume / Issue

  • 4 / 11

Start / End Page

  • 2259 - 2269

PubMed ID

  • 25236733

Pubmed Central ID

  • PMC4232551

Electronic International Standard Serial Number (EISSN)

  • 2160-1836

Digital Object Identifier (DOI)

  • 10.1534/g3.114.012922


  • eng

Conference Location

  • England