Techniques for quantifying phytoplankton biodiversity.

Journal Article (Review)

The biodiversity of phytoplankton is a core measurement of the state and activity of marine ecosystems. In the context of historical approaches, we review recent major advances in the technologies that have enabled deeper characterization of the biodiversity of phytoplankton. In particular, high-throughput sequencing of single loci/genes, genomes, and communities (metagenomics) has revealed exceptional phylogenetic and genomic diversity whose breadth is not fully constrained. Other molecular tools-such as fingerprinting, quantitative polymerase chain reaction, and fluorescence in situ hybridization-have provided additional insight into the dynamics of this diversity in the context of environmental variability. Techniques for characterizing the functional diversity of community structure through targeted or untargeted approaches based on RNA or protein have also greatly advanced. A wide range of techniques is now available for characterizing phytoplankton communities, and these tools will continue to advance through ongoing improvements in both technology and data interpretation.

Full Text

Duke Authors

Cited Authors

  • Johnson, ZI; Martiny, AC

Published Date

  • January 2015

Published In

Volume / Issue

  • 7 /

Start / End Page

  • 299 - 324

PubMed ID

  • 25251272

Electronic International Standard Serial Number (EISSN)

  • 1941-0611

International Standard Serial Number (ISSN)

  • 1941-1405

Digital Object Identifier (DOI)

  • 10.1146/annurev-marine-010814-015902

Language

  • eng