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Analyzing transcription dynamics during the budding yeast cell cycle

Publication ,  Journal Article
Leman, AR; Bristow, SL; Haase, SB
Published in: Methods in Molecular Biology
2014

Assaying global cell cycle-regulated transcription in budding yeast involves extracting RNA from a synchronous population and proper normalization of detected transcript levels. Here, we describe synchronization of Saccharomyces cerevisiae cell populations by centrifugal elutriation, followed by the isolation of RNA for microarray analysis. Further, we outline the computational methods required to directly compare RNA abundance from individual time points within an experiment and to compare independent experiments. Together, these methods describe the complete workflow necessary to observe RNA abundance during the cell cycle. © Springer Science+Business Media New York 2014.

Duke Scholars

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

2014

Volume

1170

Start / End Page

295 / 312

Publisher

Humana Press Inc.

Related Subject Headings

  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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Chicago
ICMJE
MLA
NLM
Leman, A. R., Bristow, S. L., & Haase, S. B. (2014). Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology, 1170, 295–312. https://doi.org/10.1007/978-1-4939-0888-2-14
Leman, A. R., S. L. Bristow, and S. B. Haase. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology 1170 (2014): 295–312. https://doi.org/10.1007/978-1-4939-0888-2-14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology. 2014;1170:295–312.
Leman, A. R., et al. “Analyzing transcription dynamics during the budding yeast cell cycle.” Methods in Molecular Biology, vol. 1170, Humana Press Inc., 2014, pp. 295–312. Scopus, doi:10.1007/978-1-4939-0888-2-14.
Leman AR, Bristow SL, Haase SB. Analyzing transcription dynamics during the budding yeast cell cycle. Methods in Molecular Biology. Humana Press Inc.; 2014;1170:295–312.

Published In

Methods in Molecular Biology

DOI

ISSN

1064-3745

Publication Date

2014

Volume

1170

Start / End Page

295 / 312

Publisher

Humana Press Inc.

Related Subject Headings

  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences