Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections--needs, advances, and future prospects.

Published

Journal Article (Review)

Respiratory tract infections rank second as causes of adult and paediatric morbidity and mortality worldwide. Respiratory tract infections are caused by many different bacteria (including mycobacteria) and viruses, and rapid detection of pathogens in individual cases is crucial in achieving the best clinical management, public health surveillance, and control outcomes. Further challenges in improving management outcomes for respiratory tract infections exist: rapid identification of drug resistant pathogens; more widespread surveillance of infections, locally and internationally; and global responses to infections with pandemic potential. Developments in genome amplification have led to the discovery of several new respiratory pathogens, and sensitive PCR methods for the diagnostic work-up of these are available. Advances in technology have allowed for development of single and multiplexed PCR techniques that provide rapid detection of respiratory viruses in clinical specimens. Microarray-based multiplexing and nucleic-acid-based deep-sequencing methods allow simultaneous detection of pathogen nucleic acid and multiple antibiotic resistance, providing further hope in revolutionising rapid point of care respiratory tract infection diagnostics.

Full Text

Duke Authors

Cited Authors

  • Zumla, A; Al-Tawfiq, JA; Enne, VI; Kidd, M; Drosten, C; Breuer, J; Muller, MA; Hui, D; Maeurer, M; Bates, M; Mwaba, P; Al-Hakeem, R; Gray, G; Gautret, P; Al-Rabeeah, AA; Memish, ZA; Gant, V

Published Date

  • November 2014

Published In

Volume / Issue

  • 14 / 11

Start / End Page

  • 1123 - 1135

PubMed ID

  • 25189349

Pubmed Central ID

  • 25189349

Electronic International Standard Serial Number (EISSN)

  • 1474-4457

International Standard Serial Number (ISSN)

  • 1473-3099

Digital Object Identifier (DOI)

  • 10.1016/s1473-3099(14)70827-8

Language

  • eng